Detection of superparamagnetic nanoparticles for immunoassays

Benjamin Garlan (1) , Amine Rabehi (1) , Guillaume Perry (1, 2) , Kieu Ngo (3) , Hamid Kokabi (1, 2)

1 L2E - Laboratoire d'Electronique et Electromagnétisme
2 GeePs - Laboratoire Génie électrique et électronique de Paris
3 LISE - Laboratoire Interfaces et Systèmes Electrochimiques

We develop a completely integrated Lab-on-Chip (LoC) for easy, rapid and cost-effective immunoassays. The pathogen sensing system is composed of a microfluidic channel surrounded by planar microcoils which are responsible for the emission and the detection of magnetic fields. The system allows the detection and quantification of superparamagnetic beads used for immunoassays in a “sandwich” antigen-antibody configuration. We successfully tested this device with different concentrations of nanoparticles and determine the limit of detection of the prototype. These results are promising and are a step toward the creation of a portable pathogen sensing device.

Mots clés: Lab-on-chip; magnetic detection; pathogen sensing; superparamagnetic nanoparticles; in vitro diagnostic device; microfluidics

Domaines: Automatique / Robotique; Electromagnétisme; Traitement du signal et de l'image [eess.SP]; Electronique

Benjamin Garlan, Amine Rabehi, Guillaume Perry, Kieu Ngo, Hamid Kokabi. Detection of superparamagnetic nanoparticles for immunoassays. Journées d'Etude sur la TéléSanté, Sorbonne Universités, May 2019, Paris, France. ⟨hal-02161041⟩